Accurate quantitative phase imaging using generalized phase contrast

Accurate quantitative phase imaging through telecentric digital holo- graphic microscopy

The use of non-telecentric imaging systems in quantitative phase digital holographic microscopy introduces strong inaccuracies. We show that even negligible errors in the radius and center of curvature of the remaining quadratic phase factor introduce big errors in the numerical phase measurements. The errors depend on the position of the object in the field-of-view. However, when a telecentric...

Quantitative Phase Imaging using Multi-wavelength Optical Phase Unwrapping

Flip angle optimization for quantitative phase contrast MR imaging

Background PC-MRI is a noninvasive imaging technique used to measure the velocity of flowing blood in a particular blood vessel with flexible spatial and temporal resolution [1,2]. It is considered the clinical “gold standard” for quantification of blood flow. PC-MRI boasts a variety of established applications in quantifying cardiovascular function and hemodynamics. In particular, this techniq...

Quantitative X-ray phase contrast waveguide imaging of bacterial endospores1

Quantitative waveguide-based X-ray phase contrast imaging has been carried out on the level of single, unstained, unsliced and freeze-dried bacterial cells of Bacillus thuringiensis and Bacillus subtilis using hard X-rays of 7.9 keV photon energy. The cells have been prepared in the metabolically dormant state of an endospore. The quantitative phase maps obtained by iterative phase retrieval us...

Nonlinear self-calibrated phase-contrast correction in quantitative cardiac imaging

Background Residual background phase in cardiac phase-contrast (PC) imaging introduces velocity errors that bias quantitative flow measurements [1]. While the bias can be offset using static phantoms [2], improved workflow is realized if self-calibrated correction is performed by fitting the phase of static tissue from the in vivo images. However, the residual phase can be nonlinear in space an...